Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit (Anti-H2AXS139p); IgG fraction of antiserum, buffered aqueous solution; Suitable for western blot; Anti-phospho-Histone H2AX (p(Ser139M)) antibody is suitable for use in western blot (1:1,000 using whole cell extra

I am a PhD scholar working with HL-60 cells and I am trying to induce DNA damage to them and detect gamma H2AX proteins (15kDa) by western blotting using a well-documented toxin, Arsenic. However, instead of seeing a band at the expected 15kDA region, I've detected nothing after repeating the experiment several times.

No cross reactivity with other phosphorylated histones. Immunogen: A phospho-peptide corresponding to Phospho-Histone H2AX (Ser139). Application: Western Blot (WB) Immunocytochemistry (ICC) ELISA Compare Anti-gammaH2ax Antibody Products from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. 2011-06-01 · Western blot analysis.

Gamma h2ax western blot

Abcam H2ax antibody. select up to five products for comparison. gamma H2A.X (phospho S139) antibody [3F2] Abcam. catalog: ab22551.

(C) Effect of γ-H2AX down-regulation on capillary-like tube 19 Jun 2015 Analysis of H2AX phosphorylation by Western Blot.

Bethyl Laboratories Anti-Phospho-gamma-H2AX (Ser139) Polyclonal, Catalog # A300-081A. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC) and Immunohistochemistry (IHC) applications. This antibody reacts with Human, Mouse samples. Supplied as 100 µL purified antibody (1 mg/mL).

Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (ELISA) and Luminex (LUM) applications. This antibody reacts with All, Human, Mouse, Rat samples. Supplied as 100 µg purified antibody (1 mg/mL). The attached is a γH2AX western blotting result.293T cells were treated with etoposide or DMSO (control),then cells were lysed in RIPA buffer with sonication.0.2 nitrocelluose membrane was used Western blot - Anti-gamma H2A.X (phospho S139) antibody (ab11174) All lanes : Anti-gamma H2A.X (phospho S139) antibody (ab11174) at 0.04 µg/ml Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cells treated with 100 µM etoposide, whole cell lysate The attached is a γH2AX western blotting result.293T cells were treated with etoposide or DMSO(control),then cells were lysed in RIPA buffer with sonication.0.2 nitrocelluose membrane was used Western blot and immunocytochemical analyses with the anti-gamma-H2AX antibody revealed that H2AX is phosphorylated at M phase in HeLa cells.

Group 1 starts experiment, other groups learn to score chromosomal aberration/micronuclei/γ-. H2AX and Western Blot (Lab E224). Mats. Munira K. We. CELOD.

Gamma H2AX is the phosphorylated form 2009-12-10 Histone H2AX is a 14 kDa ubiquitous member of the H2A histone family that contains an evolutionarily conserved SQ motif at the C-terminus in eukaryotes. Serine 139 within this motif becomes rapidly phosphorylated by ATM and ATR 754 gamma H2AX antibodies from 38 antibody suppliers Click each gamma H2AX antibody that interests you to view the full gamma H2AX antibody datasheet. Western Blot: Close Phospho-gamma H2AX pSer139 Antibody Product Specific Information General Information PA1-25001 detects gamma H2A.X (phospho S139) from human and mouse samples. PA1-25001 has been successfully used in Immunoflourescence, and Western blot procedures. By Western Blot a … Anti-H2AX Antibody Products. Listed below are anti-H2AX antibodies from multiple suppliers.

Furthermore, the assays commonly used for γ-H2AX detection utilize laborious and low-throughput microscopy-based methods. We describe here an ELISA assay that Anti-H2AX pS139 antibody has been tested for use in ELISA and by western blot.
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PA1-25001 has been successfully used in Immunoflourescence, and Western blot procedures. By Western Blot a … Anti-H2AX Antibody Products. Listed below are anti-H2AX antibodies from multiple suppliers. H2AX is a reported alias name for the human gene H2AFX, or 'H2A histone family member X'. The 143-amino acid protein is a member of the Histone H2A family.

Phosphorylated H2AX (also termed, gamma-H2AX) functions to recruit and localize DNA repair proteins or cell cycle checkpoint factors to the DNA-damaged sites. In this way, phosphorylated H2AX promotes DNA repair and maintains genomic stability and thus helps prevent oncogenic transformations.
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imunofluorescência revelou maior indução de células positivas para γ-H2AX na linhagem LN18 Western Blot e quantificadas pelo programa Gel Pro Analyzer

Immunocytochemistry of HeLa cells using gamma H2AX Rabbit mAb RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green). 2010-03-06 · Background When DNA double-strand breaks (DSB) are induced by ionizing radiation (IR) in cells, histone H2AX is quickly phosphorylated into γ-H2AX (p-S139) around the DSB site. The necessity of DNA-PKcs in regulating the phosphorylation of H2AX in response to DNA damage and cell cycle progression was investigated. Results The level of γH2AX in HeLa cells increased rapidly with a peak level Western blot - Anti-gamma H2A.X (phospho S139) antibody (ab11174) All lanes : Anti-gamma H2A.X (phospho S139) antibody (ab11174) at 0.04 µg/ml Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) cells treated with 100 µM etoposide, whole cell lysate DNA double strand break (DSB) are leading to the early phosphorylation of Histone H2AX. Phosphorylated Gamma-H2AX is the first step in the recruitment of the DNA repair protein at the foci site. They are consequently very good biomarkers of DNA double strand break as well as check point for cell cycle arrest following DSB. Measuring gamma-H2AX phosphorylation has become a gold standard in DNA H2AX (pS139) Pure N1-431 100ug.